Contact an Account ManagerSimultaneous LC determination of 17 of the major monoamine neurotransmitters precursors and metabolites
Summary
Type: HPLC
Application #: 102110
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: coulometric: guard cell +1.20V, first detector cell +0.20V, second detector cell (coupled to the integrator) +0.80V
Conditions: eluent: 0.1mol/l citrate buffer, 0.3mmol/l Na2EDTA, 0.293mmol/l octyl sulphate and acetonitrile - water (6.3 : 93.7, v/v), pH 2.35,
flow rate 1.2ml/min,
temperature 25°C
Authors: Wester P
Source: J Chromatogr 415 (1987) 261 - 274 275 - 288
Details
Legend: Chromatogram of 15pmol of standards, 20µl injected;
DHPG = dihydroxyphenylethylene glycol,
VMA = vanillylmandelic acid,
MHPG = 3-methoxy-4-hydroxyphenylethylene glycol,
DOPAC = dihydroxyphenylacet